The Extract from Punica Granatum (Pomegranate) Leaves Promotes Apoptosis and Impairs Metastasis in Prostate Cancer Cells

DENG Yuan-le, LI Ya-li, ZHENG Ting-ting. et al


To investigate the effects of pomegranate leaves extract (PLE) on proliferation, apoptosis and metastasis of prostate cancer cells. Methods The proliferation of TRAMP-Cl, DU 145, PC3 prostate cancer cells treated with different concentrations of PLE (final mass concentrations were 12. 5, 25, 50, 100, 200 jxg/mL, respectively) for different time (24, 48, 72 h) was detected by MTT assay. Colony formation assay was performed to verify the long-term effects of PLE on the proliferation of DU 145 and PC3 cells. After being treated with PLE for 48 h, Hoechst-33258 staining was used to observe the changes in the nucleus, the cell apoptotic rate was detected by flow cytometry, and wound-healing migration assay was perform to test the change of migration. Results In comparison with the control group, PLE in the range of 12. 5-200 /ig/mL had a certain inhibitory effect on the proliferation of TRAMP-Cl, DU145 and PC3 cells (P<0. 05). In the range of 6. 25- 100 цg/mL, the number of colony formation of DU145 and PC3 was significantly reduced(P<0. 01). After PLE treated for 48 h,the apoptotic features of nuclear fragmentation and the formation apoptotic body was observed in PC3. With the increase of concentration, the apoptotic rate increased gradually (P<0. 05), and the ability of cells to migrate to the scratch area was significantly weaker than the control group (P<0. 01). Conclusion PLE has effect on proliferation, apoptosis and metastasis of prostate cancer cells.


Keywords: Pomegranate leaves extract (PLE) Prostate cancer PC3 Apoptosis


Full Text:



SIEGEL RL, MILLER KD, JEMAL A. Cancer statistics. С A Cancer J Clin, 2016, 66( 1): 7-30.

CHEN W, ZHENG R, BAADE PD. Et al. Cancer statistics in China. CA Cancer J Clin.2016.66(2): 115-132.

ISMAIL T, SESTILI P. AKHTAR S. Pomegranate peel and fruit extracts: a review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol, 2012, 143(2); 397- 405.

LI Y. YE T, YANG F, et al. Punicagranatum (pomegranate) peel extract exerts potent antitumor and anti¬metastasis activity in thyroid cancer. RSC Advances, 2016 ,6 (87): 84523-84535 [2017-03-21]. http://pubs. rsc. org/-/ content/articlehtml/2016/ra/c6ral3167k. doi: 10. 1039/C6RA13167K.

VROEGRIJK IO, VAN DIEPEN JA, VAN DEN BERG S, et al. Pomegranate seed oil. a rich source of punicic acid, prevents diet-induced obesity and insulin resistance in mice. Food Chem Toxicol,2011 ,49(6): 1426-1430.

LONG Z. CHEN B, LIU Q. et al. The reverse-mode NCX1 activity inhibitor KB-R7943 promotes prostate cancer cell death by activating the JNK pathway and blocking autophagicflux. Oncotarget ,2016,7(27) :42059-42070.

SALEEM M. ADHAMI VM, ZHONG W, et al. A novel biomarker for staging human prostate adenocarcinoma; overexpression of matriptase with concomitant loss of its inhibitor, hepatocyte growth factor activator inhibitor-1. Cancer Epidemiol Biomarkers Prev,2006,15(2):217-227.

TOSHIYA K. TESTUYA T, AKIRA H. et al. Cancer chemoprevention through the induction of apoptosis by natural compounds. Journal of Biophysical Chemistry,2012,3 (2 ); 156-173 [2017-03-21 ]. http: / / file, scirp. org/Html/7- 7100133_ 19431. htm. doi;10. 4236/jbpc. 2012. 32018.

LI Y, YANG F, ZHENG W, et al. Punicagranatum, ( pomegranate ) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in non-small cell lung cancer in vitro. Biomed Pharmacother, 2016, 80: 227-235 [ 2017-03-21 ]. https; //doi. org/10. 1016/j. biopha. 2016. 03. 023.


  • There are currently no refbacks.