The Anti-virus Effect of AY358935 Gene on Vesicular Stomatitis Virus and the Mechanism Study

LI Guang-ping, KE Jian-long, LÜ Pan-pan, ZHU Xi, WANG Cui, WANG Jin-yang, SHEN Xian-ying, XIONG Shao-q

Abstract

To explore the anti-virus effect of AY358935 gene cloned by our research team on vesicular stomatitis virus (VSV), and studytheanti-virus mechanism.  Methods  HEK293 cells were stably transfected by the AY358935 gene recombinant plasmid pcDNA3.1-AY358935 or pcDNA3.1 blank plasmid respectively. Then VSV was added into the cell wells to infect the above cells at the multiplicity of infection (MOI) of 0.001. The virus titers in the liquid supernatant of the above three groups of cells were detected on different time, and the mortality of cells of each group was tested with trypan blue exclusion test at 24 h post VSV infection. Total RNA was extracted from the cells that stably transfected with target gene for the whole genome-wide cDNA microarray analysis.  Results  ① Virus titer:The virus titer in the liquid supernatant of pcDNA-3.1-AY358935 transfection cells group was obviously lower than those in pcDNA-3.1 transfection cell group and blank control cell group at 12 h post infection. The virus titerin the liquid supernatant of three groups were (7.16±2.33)×105 PFU/mL, (6.25±2.05)×106 PFU/mL and (7.75±2.54)×106 PFU/mL respectively at 18 h post infection. At that time, the virus titerin the liquid supernatant of pcDNA3.1-AY358935 group was nearly 10 times lower than those of other two groups (P < 0.01). ②Mortality of cells:The cell mortality of pcDNA3.1-AY358935 group, pcDNA3.1 group and blank group were (35.00±6.68)%, (78.33±15.03)% and (83.34±14.98)% respectively at 24 h post infection.The cell mortality of pcDNA3.1-AY358935 group was significantly decreased comparing with other two groups (P < 0.01). ③Result of genes chip analysis: compared with pcDNA3.1 group, 30 cell genes were up-regulated by more than 3 times in pcDNA3.1-AY358935 group. Among them, the proportion of interferon-activating gene, interferon-effect gene, cytokine and chemokine was 27%, 17%, and 20%, respectively.  Conclusion  AY358935 gene hasan anti-VSV effect, and its anti-virus mechanism may involve the interferon-associated natural immune response.

 

Keywords: AY358935 gene, VSV, Interferon


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References


GNANADURAI CW. FU ZF. CXCL10 and blood-brain barrier modulation in rabies virus infection. J Oncotarget, 2016.7 (10):10694-10695.

ZHAO A, LU W, DE LE. Functional synergism of human defensin 5 and human defensin 6. Biochem Biophys Res Commun,2015,467(4) :967-972.

CURRIE SM. FINDLAY EG, MCFARLANE AJ, et al. Cathelicidins have direct antiviral activity against respiratory syncytial virus in vitro and protective function in vivo in mice and humans. J Immunol,2016,196(6) :2699-2710.

PEEL E. CHENG Y, DJORDJEVIC JT, et al. Cathelicidins in the Tasmanian devil (Sarcophilus harrisii). Sci Rep,2016, 6:35019.

REBHANDLS, HUEMER M, GREIL R, et at. AID/ APOBEC deaminases and cancer. Oncoscience, 2015 , 2 ( 4 ); 320-333.

LEI Q, GAO B, ZHANG D. Design and preparation of matrine surface-imprinted material and studies on its molecule recognition selectivity. J Biomater Sci Polym Ed, 2016, 27 (1) : 1-21.

SAFA A, RASHIDINEJAD HR, KHALILI M, et al. Higher circulating levels of chemokines CXCL10, CCL20 and CCL22 in patients with ischemic heart disease. Cytokine, 2016,83: 147-157[2018-10-10]. https;//doi. org/10. 1016/j. cyto. 2015.04.006.

GRAWENHOFF J, ENGELMAN AN. Retroviral integrase protein and intasome nucleoprotein complex structures. World J Biol Chem,2017,8( 1):32-44.

YIN Y. LIU W. DAI Y. S0CS3 and its role in nassociated diseaseas. Hum Immunol ,2015,76( 10); 775-780.

CHOW KT, GALE MJ. SnapShot; interferon signaling. Cell,2015, 163( 7): 1808el [2018-10-10]. https://doi. org/ 10. 1016/j. cell. 2015. 12.008.

2012,9( 15) ;84-86. HUANG Y, HUANG X. CAI J, el at. Identification of orange-spotted grouper ( Epinephelus coioides ) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus. Fish Shellfish Immunol, 2015, 42 (2):345-352.

HENKES LE, PRU JK, ASHLEY RL, et al. Embryo mortality in Isgl5~/_ mice is exacerbated by environmental stress. Biol Reprod.2015, 92( 2): 36[2018-10-10]. https:// doi. org/10. 1095/biolreprod. 114. 122002.

DONNELLY RP, KOTENKO SV. Interferon-lambda:a new addition to an old family. J Interferon Cytokine Res,2010,30 (8);555-564.

GIBBERT K, SCHLAAKJ F, YANG D. IFN-alpha subtypes;distinct biological activities in anti-viral therapy. Br J Pharmacol, 2013,168(5); 1048-1058.

DONNELLY RP. KOTENKO SV. Interferon-lambda:a new addition to an old family. J Interferon Cytokine Res,2010,30 8);555-564.

DE VEER MJ, HOLKO M, FREVEL M. et al. Functional classification of interferon-stimulated genes identified using microarrays. J Leukoc Biol,2001,69(6) ;912-920.

SCHNEIDER WM. CHEVILLOTTE MD, RICE CM. Interferon- stimulated genes:a complex web of host defenses. Annu Rev Immunol,2014,32:513-545[2018-10-10 . https;// doi. org/10. 1146/annurev-immunol-032713-120231.

CHEN Z, MARK C, TIEN YH, et al. Interferon-induced ISO 15 pathway: an ongoing virus-host battle. Trends Microbiol,2013,21(4); 181-186.


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