LINC ROR from Hepatocarcinoma Cell-derived Exosomes Modulates Inflammation in Human Macrophages

LI Xue, LI Nan

Abstract

To explore the regulatory function of long intergenic non-coding RNA LINC ROR that contained within hepatocarcinoma cells-derived exosomes. MethodsThe physiological appearance of exosomes isolated from PLC/PRF/5 cells was examined by using differential ultracentrifugation and the images were captured by transmission electron microscopy. The internalization of exosomes by THP-1 cells was examined via laser-scanning confocal microscopy. The expression of LINC ROR was analyzed through qRT-PCR. The expression of interleukin （IL）-1β was examined by ELISA. ResultsExosomes were found spherical and the diameters mainly distribute around 100 nm. LINC ROR can be transferred by tumor derived exosomes to macrophages. Exosomes isolated from PLC/PRF/5 cells significantly expressed higher levels of LINC ROR. Suppression of LINC ROR by siRNA significantly increased the release of pro-inflammatory cytokine (IL-1β) in THP-1 cell upon LPS stimulation. ConclusionLINC ROR from hepatocarcinoma cell-derived exosomes plays an important role in regulating the inflammation of macrophages.

 

Keywords: ExosomesLong intergenic non-coding RNA, LINC ROR, Hepatocarcinoma, Macrophages 

 

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