Optimal Timing of Moxibustion Intervention for Anti-inflammatory and Analgesic Effects Based on a Chronic Inflammatory Pain Model
Abstract
Based on a complete Freund's adjuvant (CFA)-induced chronic inflammatory pain model, we compared and analyzed the differences in anti-inflammatory and analgesic effects of moxibustion intervention initiated at different timepoints, aiming to identify the optimal timing for moxibustion intervention. The goal is to establish standardized intervention protocols for basic research on the anti-inflammatory and analgesic effects of moxibustion.
Methods Male C57BL/6 mice were randomly divided into 3 groups based on the moxibustion initiation timepoints of 4, 7, and 10 d after modeling. Then, the mice in each group were randomly assigned to 3 subgroups, including a control group, a model group, and a moxibustion group, with 8 mice in each subgroup. Chronic inflammatory pain was induced by injecting 20 μL of CFA into the sole of the right hind paw. Moxibustion applied at the "Zusanli" acupoint for 30 minutes started on the 4th, 7th, and 10th days after modeling, and the intervention continued for 7 days. The latency of paw withdrawal to thermal radiation was measured to evaluate the pain threshold before modeling, after modeling, and on the 1st, 4th, and 7th days of treatment. Foot volume was measured to assess toe swelling before modeling, after modeling, and on the 1st and 7th days of treatment.
Results Compared with the control group, the model group exhibited a reduced pain threshold (P < 0.0001) and increased paw volume (P < 0.0001). Compared with the model group, the subgroups receiving moxibustion intervention initiated on the 4th, 7th, and 10th days post-modeling exhibited an increased pain threshold (P < 0.05, P < 0.0001). However, the paw volume of the subgroups receiving moxibustion intervention initiated on the 4th day post-modeling increased (P < 0.0001), while those of the subgroups receiving moxibustion intervention initiated on the 7th and 10th days post-modeling decreased (P < 0.0001). Among the intervention subgroups receiving moxibustion initiated on days 4, 7, and 10, the day 7 intervention-initiating subgroup showed significant increase in pain threshold (P < 0.05, P < 0.0001), and the day 7 and day 10 intervention-initiating subgroups showed significantly reduced paw volume (P < 0.0001).
Conclusion Considering both the analgesic and anti-inflammatory effects of moxibustion, day 7 post-modeling may be the optimal time for moxibustion to achieve effective anti-inflammatory and analgesic outcomes.
Keywords: Moxibustion, Intervention timing, Chronic inflammatory pain
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WU H G, WENG Z J, LIU H R, et al. Analgesic and anti-inflammatory effects of moxibustion on immune-related disease. World Chin Med, 2016, 11(12): 2505-2514. doi: 10.3969/j.issn.1673-7202.2016.12.001.
CHOI T Y, LEE M S, KIM J I, et al. Moxibustion for the treatment of osteoarthritis: an updated systematic review and meta-analysis. Maturitas, 2017, 100: 33-48. doi: 10.1016/j.maturitas.2017.03.314.
KIM T H, KIM K H, KANG J W, et al. Moxibustion treatment for knee osteoarthritis: a multi-centre, non-blinded, randomised controlled trial on the effectiveness and safety of the moxibustion treatment versus usual care in knee osteoarthritis patients. PLoS One, 2014, 9(7): e101973. doi: 10.1371/journal.pone.0101973.
ZHAO W W, YU B F, WANG Q F, et al. Meta-analysis of clinical efficacy and adverse events of moxibustion on treating ulcerative colitis. J Liaoning Univ Tradit Chin Med, 2023, 25(10): 74-79. doi: 10.13194/j.issn. 1673-842x.2023.10.015.
KIM J H, KIM H K, PARK Y I, et al. Moxibustion at ST36 alleviates pain in complete freund's adjuvant-induced arthritic rats. Am J Chin Med, 2006, 34(1): 57-67. doi: 10.1142/S0192415X06003631.
ZHENG B, HU L, SONG X, et al. Analgesic effect of different moxibustion durations in rheumatoid arthritis rats. J Tradit Chin Med, 2014, 34(1): 90-95. doi: 10.1016/s0254-6272(14)60060-1.
ZUO C Y, GOU C Y, ZHANG C S, et al. Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain. Heliyon, 2023, 9(7): e17765. doi: 10.1016/j.heliyon.2023.e17765.
ZENG L, GUO J, DU P, et al. Transcriptome sequencing reveals core regulation modules and gene signatures of Zusanli acupoints in response to different moxibustion warm stimulation in adjuvant arthritis rat. Hereditas, 2022, 159(1): 15. doi: 10.1186/s41065-022-00221-4.
GHASEMLOU N, CHIU I M, JULIEN J P, et al. CD11b+Ly6G- myeloid cells mediate mechanical inflammatory pain hypersensitivity. Proc Natl Acad Sci U S A, 2015, 112(49): E6808-E6817. doi: 10.1073/pnas. 1501372112.
WU H G, MA X P, LIU H R, et al. Thinking of moxibustion research. Mod Tradit Chin Med Mater Medica-World Sci Technol, 2016, 18(3): 355-360. doi: 10.11842/wst.2016.03.005.
WU H G , WU L. Research progress and key scientific problems of traditional Chinese moxibustion. Acupunct Herbal Med, 2024, 4(1): 16-18. doi: 10.1097/HM9.0000000000000091.
SUN Z L, XU X, DU S Z, et al. Moxibustion for treating rheumatoid arthritis: a systematic review and meta-analysis of randomized controlled trials. Eur J Integr Med, 2014, 6(6): 621-630. doi: 10.1016/j.eujim.2014.08.002.
SHEN B, SUN Q, CHEN H, et al. Effects of moxibustion on pain behaviors in patients with rheumatoid arthritis: a meta-analysis. Medicine (Baltimore), 2019, 98(30): e16413. doi: 10.1097/MD.0000000000016413.
MA X R, CHAI Z, LIU F Y, et al. Moxibustion in the treatment of keen osteoarthritis: a systematic review and Meta analysis. Shanghai J Tradit Chin Med, 2017, 51(9): 19-23. doi: 10.16305/j.1007-1334.2017.09.005.
YUAN T, XIONG J, WANG X, et al. The effectiveness and safety of moxibustion for treating knee osteoarthritis: a PRISMA compliant systematic review and meta-analysis of randomized controlled trials. Pain Res Manag, 2019, 2019: 2653792. doi: 10.1155/2019/2653792.
WANG D, WANG Q, WANG Y, et al. Effects of acupuncture and moxibustion on ulcerative colitis: an overview of systematic reviews. Heliyon, 2024, 10(6): e27524. doi: 10.1016/j.heliyon.2024.e27524.
BURMA N E, LEDUC‐PESSAH H, FAN C Y, et al. Animal models of chronic pain: advances and challenges for clinical translation. J Neurosci Res, 2017, 95(6): 1242-1256. doi: 10.1002/jnr.23768.
CHILLINGWORTH N L, DONALDSON L F. Characterisation of a freund's complete adjuvant-induced model of chronic arthritis in mice. J Neurosci Methods, 2003, 128(1/2): 45-52. doi: 10.1016/s0165-0270(03) 00147-x.
LARSON C M, WILCOX G L, FAIRBANKS C A. The study of pain in rats and mice. Comp Med, 2019, 69(6): 555-570. doi: 10.30802/AALAS-CM-19-000062.
ZHOU W, LEI R, ZUO C, et al. Analgesic effect of moxibustion with different temperature on inflammatory and neuropathic pain mice: a comparative study. Evid Based Complement Alternat Med, 2017, 2017: 4373182. doi: 10.1155/2017/4373182.
ZUO C Y, LV P, ZHANG C S, et al. Ipsi- and contralateral moxibustion generate similar analgesic effect on inflammatory pain. Evid Based Complement Alternat Med, 2019, 2019: 1807287. doi: 10.1155/2019/ 1807287.
YIN H Y, FAN Y P, LIU J, et al. Purinergic ATP triggers moxibustion-induced local anti-nociceptive effect on inflammatory pain model. Purinergic Signal, 2023, 19(1): 5-12. doi: 10.1007/s11302-021-09815-5.
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