Expression of ACTN2, α-actin and TNNT2 in Rat Bone Marrow-derived Mesenchymal Stem Cells Induced by Low Frequency Pulsed Electromagnetic Fields

LI Kai, JIANG Jian, YAO Xiao-lin, QIU Fei-yuan, YAN Zhong-qin, WU Wen-chao, LIU Xiao-jing, LI Liang

Abstract

To study the expression of Actinin, alpha 2 (ACTN2), α-actin and Troponin T type 2 (TNNT2) in rat bone marrow-derived mesenchymal stem cells (rBMSCs) induced by low frequency pulsed electromagnetic fields (PEMFs). Methods The rBMSCs were isolated by adherence method and the third-generation of the rBMSCs were randomly divided into PEMFs groups, 5-Azacytidine groups (5-Aza), PEMFs +5-Aza group and control groups. PEMFs groups with complete medium were exposed to 50 Hz, 1 mT PEMFs for 30 min every day, lasting for 10 d, 15 d and 20 d, respectively. 5-Aza groups were induced by 10 μmol/L 5-Aza for 1 day, then the medium was changed to complete medium without 5-Aza. PEMFs +5-Aza group were firstly induced by 10μmol/L 5-Aza for 1 day, and then were exposed to PEMFs according to the way of PEMFs group. And control groups were only cultured with complete medium. rBMSCs' growth status and morphological features were observed by inverted phase microscope. The mRNA expressions of ACTN2 and TNNT2 were determined by real-time fluorescent quantitation PCR. And the protein expression of the ACTN2, α-actin and TNNT2 were detected with Western blot method. Results The result showed that the mRNA expression level of the ACTN2 and TNNT2 and the protein expression level of the ACTN2, α-actin and TNNT2 were significantly higher in the PEMFs group, 5-Aza group and PEMFs+5-Aza group when compared with the control group. Conclusion PEMFs may induce rBMSCs differentiation into cardiomyocyte-like cells in vitro.

 

Keywords: Actinin, alpha 2, α-actin, Troponin T type 2, Pulsed electromagnetic fields

 

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References


Kochanek КD, Kirmeyer SE. Martin JA, et al. Annual summary of vital statistics: 2009. Pediatrics, 2012; 129 ( 2 ): 338-348.

Meisner M, Adina H. Schmidt J. Correlation of procalcitonin and C-reactive protein to inflammation, complications, and outcome during the intensive care unit course of multiple- trauma patients. Crit Care,2006; 10(1); Rl.

Rivers E, Nguyen B, Havstad S, et al. Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med.2001 ;345( 19): 1368-1377.

Goldstein B, Giroir B, Randolph A, et al. International pediatric sepsis consensus conference; definitions for sepsis and organ dysfunctionin pediatrics. Pediatr Crit Care Med, 2005; 6

Tepas JJ, Mollitt DL, Talbert JL, et al. The pediatric trauma score as a predictor of injury severity in the injured child. J Pediatr Surg,1987 ;22( 1); 14-18.

Assicot M, Gendrel D. Carsin H, et al. High serum procalcitonin concentrations in patients with sepsis and infection. Lancet , 1993;341(8844):515-518.

Mimoz O. Benoist JF, Edouard AR, et al. Procalcitonin and C-reactive protein during the early posttraumatic systemic inflammatory response syndrome. Intensive Care Med. 1998;24(2):185-188.

Wanner GA, Keel M, Steckholzer U. Relationship between procalcitonin plasma levels and severity of injury, sepsis, organ failure, and mortality in injured patients. Crit Care Med.2000; 28(4):950-957.

Hensler T, Sauerland S, Lefering R, et al. The clinical value of procalcitonin and neopterin in predicting sepsis and organ failure after major trauma. Shock,2003;20(5) ;420-426.

Becker KL, Snider R, Nylen ES. Procalcitonin assay in systemic inflammation, infection, and sepsis; clinical utility and limitations. Crit Care Med.2008;36(3);941-952.

Uzzan B, Cohen R, Nicolas P, et al. Procalcitonin as a diagnostic test for sepsis in critically ill adults and after surgery or trauma; a systematic review and meta-analysis. Crit Care Med.2006;34(7):1996-2003.

Simon L, Saint-Louis P. Amre DK .et al. Procalcitonin and C- reactive protein as markers of bacterial infection in critically ill children at onset of systemic inflammatory response syndrome. Pediatr Crit Care Med,2008;9(4):407-413.

Benoist JF. Mimoz O. Assicot M, el al. Serum procalcitonin, but not c-reactive protein, identifies sepsis in trauma patients. Clin Chem, 1998;44(8 Pt 1): 1778-1779.

Haasper C, Kalmhach M. Dikos GD, el al. Prognostic value of procalcitonin (PCT) and/or interleukin-6 (IL-6) plasma levels after multiple trauma for the development of multi organ dysfunction syndrome (MODS) or sepsis. Technol Health Care,2010;18(2):89-100.


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