Resolvin D1 Reduces Cerulein and Lipopolysaccharide-induced Severe Acute Pancreatitis in Mice

YE Dan, WANG Feng-ying , LIU Zuo-yan.et al

Abstract

To investigate the therapeutic effects of resolvin D1 (RvD1) on cerulein and lipopolysaccharide (LPS)-induced severe acute pancreatitis in mice．MethodsThe model of severe acute pancreatitis was induced by cerulein combined with LPS in mice. Mice were treated with RvD1 at a dose of 150 mg/kg for 4 h after the last injection of cerulein. Pathological changes and scores were assessed by HE staining,serum amylase and lipase levels were detected by ELISA,serum tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) levels were determined by Luminex Assay. ResultsCerulein combined with LPS successfully induced severe acute pancreatitis model in mice. RvD1 reduced the pathological changes of pancreas in severe acute pancreatitis mice,decreased the serum levels of amylase and lipase,as well as attenuated the serum levels of TNF-α and IL-6. ConclusionRvD1 can reduce the severity of severe acute pancreatitis induced by cerulein and LPS in mice．

 

Keywords: Acute pancreatitis, Resolvin D1, Cerulein, Lipopolysaccharide, Cytokine 


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