Dexamethasone Blocks Adriamycin-induced Podocytes'Mobility via Impacting Nephrin Expression

QIU Bing, GAO Xia, CUI Wei-jing.et al

Abstract

To explore how dexamethasone (Dex) directly restores kidney podocyte function in adriamycin (ADR)-induced nephrotic model and the effects of Dex on the motility of podocytes,to analyze whether nephrin is a key signal molecule in the process. MethodsThe cultured podocytes were divided into three growps: ADR treated group,ADR+Dex group,blank control group. The analyses of podocytes function were performed using scrape-wound,Transwell migration assays and FITC-BSA. Quantitative real-time PCR and Western blot were used to test the expression of nephrin. Male SD rats were used to generate ADR-induced nephrology model,and randomly divided into three groups: ADR group,ADR+Dex group and normal group. At 7 d,14 d,21 d and 28 d after ADR injection,24 h urine protein was measured as well. Podocyte foot process effacement was observed under transmission electron microscopy. ResultsPodocytes’ motility,permeability of a monolayer of podocytes incubated with FITC-BSA,the expression of nephrin were higher in ADR group than those in blank control group (P<0.05); on the contrary,the indexes above in Dex+ADR group were decreased when compared with ADR group (P<0.05). 24 h urine protein increased significantly at day 14 (vs. normal group P<0.001) and peaked at day 28 in ADR rats (vs. normal group P<0.001),whereas decreased at day 14,21 and 28 in Dex+ADR group (vs. ADR group,P<0.001). The FWP of ADR-treated rats was greater than normal group and Dex+ADR group (P<0.01). ConclusionDex impacts the expression of nephrin,relieves the enhanced motility induced by ADR and decreases urine protein level.

 

Keywords: Dexamethasone, Adriamycin, Podocytes, Motility, Nephrin 

 

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