The Effect of Surfentanyl and Fentanyl at Rhythmic Spontaneous Contraction on Isolated Rabbits Uterine Smooth Muscles
Abstract
To investigate the effects of surfentanyl and fentanyl on spontaneous contractions of isolated uterine smooth muscles of rabbits. Methods Thirty isolated uterine smooth muscles strips of rabbits with rhythmic spontaneous contraction were randomly divided into three groups:control group (n=10) fentanyl group (n=10) and surfentanyl group (n=10). The effects of fentanyl and surfentanyl on spontaneous contractions intensity, frequency and duration of isolated uterine smooth muscles of rabbits in different concentrationswere monitored. Results There were no effect on the spontaneous contraction of isolated uterine muscles of rabbits at the concentration of 1×10-8 mol/L in fentanyl group. The spontaneous contraction intensity was decreased significantly when the concentration of fentanyl more than 3×10-8 mol/L (P<0.05). The spontaneous contraction intensity, frequency and duration was inhibited more than 50% when the concentration of fentanyl more than 3×10-7 mol/L (P<0.05). And the spontaneous contraction duration was inhibited more than 50% when the concentration of surfentanyl more than 1×10-7 mol/L (P<0.05). Compared with surfentanyl group, there were significant difference on spontaneous contraction intensity when the concentration of fentanyl more than 3×10-8 mol/L (P<0.05). Difference on frequency, more than 3×10-8 mol/L (P<0.05) and difference on duration, at the concentration of 1×10-7 and 1×10-7 mol/L (P<0.05). Conclusion Fentanyl restrained the spontaneous contractions of isolated uterine smooth muscles of rabbits in concentration-dependent manner. Surfentanyl could reduce the spontaneous contractions duration on isolated uterine smooth muscles of rabbits. But there were no effect on frequency and duration.
Keywords: Opioids, Fentnayl, Surfentanyl, Uterine muscle
Full Text:
PDFReferences
Nacitarhan С, Sadan G, Kayacan N, et al. The effects of opioids, local anesthetics and adjuvants on isolated pregnant rat uterine muscles. Methods Find Exp Clin Pharmacol, 2007; 29 (4):273-276.
Douglas AJ, Clarke G, MacMillan SJ. et al. Effects of the kappa-opioid agonist U50, 488 on parturition in rats. Br J Pharmacol,1993; 109( 1) :251-258.
Shin YK, Collea JV, Kim YD. The effect of glucagons on spontaneous contractility of isolated pregnant uterine muscle. Obstet Gynecol. 1996;88(5):867.
Kayacan N, Ertugrul F. Arici G, et al. In vitro effects of opioids on pregnant uterine muscle. Adv Ther, 2007; 24 ( 2 ): 368-375.
Evron S, Ezri T. Options for systemic labor analgesia. Curr Opin Anaesthesiol,2007;20(3):181-185.
Volmanen P. Sarvela J, Akural El, et al. Intravenous remifentanil vs. epidural levobupivacaine with fentanyl for pain relief in early labour; a randomised, controlled, double-blinded study. Acta Anaesthesiol Scand,2008;52(2):249-255.
Yoo KY. Lee J, Kim HS, et al. The effects of opioids on isolated human pregnant uterine muscles. Anesth Analg,2001; 92(4):1006-1009.
Zhang X, Wang L, Xu L, et al. Effects of atractylodes macrocephala on the cytomembrane Ca2+-activated Кb currents in cells of human pregnant myometrial smooth muscles. J Huazhong Univ Sci Technolog Med Sci,2008;28 (2);200-203.
Kanaya N, ZakharyDR, Murray PA, elal. Differential effects of fentanyl and morphine on intracellular Ca2+ transients and contraction in rat ventricular myocytes. Anesthesiology, 1998; 89:1532-1542.
Andrade A, Denome S, Jiang YQ. Et al. Opioid inhibition of N-type Ca2+ channels and spinal analgesia couple to alternative splicing. Nat Neurosci,2010; 13(10): 1249-1256.
Lim ВН. СЬо BI. Kim YN, et al. Overexpression of nicotinamide N-methyltransferase in gastric cancer tissues and its potential post-translational modification. Exp Mol Med. 2006;38(5):4 5 5-4 65.
Roessler M. Rollinger W, Palme S. et al. Identification of nicotinamide N-methyltransferase as a novel serum tumor marker for colorectal cancer. Clin Cancer Res, 2005; 11 (18) ; 6550-6557.
Sartini D. Muzzonigro G. Milanese G. et al. Identification of nicotinamide N-methyltransferase as a novel tumor marker for renal clear cell carcinoma. J Urol.2006; 176(5) :2248-2254.
Xu J, Moatamed F. Caldwell JS, et al. Enhanced expression of nicotinamide N-methyltransferase in human papillary thyroid carcinoma cells. J Clin Endocrinol Metab. 2003; 88( 10): 4990- 4996.
Roessler M, Rollinger W, Palme S, et al. Identification of nicotinamide N-methyltransferase as a novel serum tumor marker for colorectal cancer. Clin Cancer Res, 2005; 11 (18) ; 6550-6557.
XuJ, Capezzone M. Xu X, et al. Activation of nicotinamide N-methyltransferase gene promoter by hepatocyte nuclear factor-1 beta in human papillary thyroid cancer cells. Mol Endocrinol, 2005; 19(2): 527-539.
Yao M, Tabuchi H, Nagashima Y, el al. Gene expression analysis of renal carcinoma; adipose differentiation-related protein as a potential diagnostic and prognostic biomarker for clear-cell renal carcinoma. J Pathol,2005;205(3) ;377-387.
Tomida M. Mikami I, Takeuchi S, el al. Serum levels of nicotinamide N-methyltransferase in patients with lung cancer. J Cancer Res Clin Oncol,2009; 135(9): 1223-1229.
Wu Y. Siadaty MS, Berens ME, el al. Overlapping gene expression profiles of cell migration and tumor invasion in human bladder cancer identify metallothionein IE and nicotinamide N-methyltransferase as novel regulators of cell migration. Oncogene,2008;27(52):6679-6689.
KimJ, Hong SJ. Lim EK, et al. Expression of nicotinamide N-methyltransferase in hepatocellular carcinoma is associated with poor prognosis. J Exp Clin Cancer Res, 2009; 16 (2); 20- 28.
D’ Andrea FP, Safwat A, Kassem M, et al. Cancer stem cell overexpression of nicotinamide N-methyltransferase enhances cellular radiation resistance. Radiother Oncol, 2011; 99 (3) ; 373-378.
Refbacks
- There are currently no refbacks.
