Expression of Nerve Growth Factor and Type 3 of Acid Sensitive Ion Channels in Rat Model of Type Ⅲ Prostatitis

ZHENG Ming-xing, LIU Xiao-dong, FAN Shi-cheng. et al

Abstract

To investigate the expressions of nerve growth factor (NGF) and acid-sensing ion channel 3 (ASIC3) in prostatic tissue of experimental rats with type Щ prostatitis. Methods Thirty SI) rats were randomly allocated into control group and experimental group. The rats in control group were subjected to pelvic and bilateral scapular subcutaneous injections of 0. 9% sodium chloride, while the rats in experimental group were given pelvic and bilateral scapular subcutaneous injections of mixed suspension of complete Freund’s adjuvant and prostatic tissue to induce autoimmune prostatitis (EAP). Tactile allodynia was quantified using Von-Frey as a measure of pelvic pain behavior. This measurement was performed on 0th, 5th, 10,h, 20,h, 30,h and 40,h day in the two groups. After that, the prostate samples were collected and processed for HE staining, while the expressions of NGF and ASIC3 were measured by immunohistochemistry and Western blot. Results Von-Frey filaments measurement showed that pelvis pain in EAP group was significantly more obvious than that in control group. HE staining found lymphocytes and neutrophils infiltrated in the prostate of EAP rats, but no inflammatory cells in the prostate of control group rats. The expressions of NGF and ASIC3 were significantly increased in EAP group when compared with control group (P<0. 01). Conclusion The expressions of NGF and ASIC3 in the prostate with EAP were significantly increased, which may be the important mediators of chronic pelvic pain.

 

Keywords: Type III prostatitis /chronic prostatitis, Chronic pelvic pain, NGF,  ASIC3,  VonFrey


Full Text:

PDF


References


ZHANG Z, LI Z. YU Q.et al. The prevalence of and risk factors for prostatitis-like symptoms and its relation to erectile dysfunctionin Chinese men. Andrology, 2015, 3 ( 6 ): 1119-1124.

KELLEHER JH, TEWARI D, MCMAHON SB. Neurotrophic factors and their inhibitors in chronic pain treatment. Neurobiol Dis,2017,97( PtB): 127-138.

SCHUHMACHER LN, SMITH ES. Expression of acid- sensing ion channels and selection of reference genes in mouse and naked molerat. Mol Brain,2016,9(1):97.

HU Y, NIU X. WANG G, et al. Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction.oxidative stress,apoptosis. and corporal fibrosis in a rat model. Andrology, 2016 , 4 ( 6 ): 1209-1216.

QUICK ML. DONE JD, THUMBIKAT P. Measurement of tactileallodynia in a murine model of bacterial prostatitis. J Vis Exp.2013,71 ;e50158. doi: 10. 3791/50158.

CHOPRA S, SAT К U N ASIV A M R, ARON M. Feasibility of roboticradical prostatectomy for medication refractory chronic pros-tatitis/chronic pelvic painsyndrome: initial results. Indian J Urol,2016,32(3):238-241.

BRESER ML,MOTRICH RD,SANCHEZ LR, et al. Chronic pelvic pain development and prostate inflammation in strains of mice with different susceptibility to experimental autoimmune prostatitis. Prostate.2017 ,77(1):94-104.

MIZUMURA K.MURASE S. Role of nerve growth factor in pain. Handb Exp Pharmacol, 2015, 227: 57-77. doi: 10. 1007/ 978-3-662-46450-2-4.

MCKELVEY L, SHORTEN GD, O’ KEEFFE GW. Nerve growth factor-mediated regulation of pain signalling and proposdnew intervention strategies inclinical pain management. J Neurochem,2013 ,124(3) :276-289.

MARRAMS, FERRU-CLEMENT R, BREUIL V, et al. Non-acidic activation of pain-related Acid-Sensing Ion Channel 3 by lipids. EMBO J ,2016,35(4) ;414-428.

CORROWK, GIRARD ВМ, VIZZARD MA. Expression andresponse of acid-sensing ion channels in urinary bladder to cyclophosphamide-induced cystitis. Am J Physiol Renal Physiol,2010, 298 ( 5 ): FI 130-F1139 [2017-01-22]. http;// ajprenal. physiology. org/content/298/5/Fl 130. long, doi: 10.1152/ajprenal. 00618. 2009.

CHANG DS, HSU E, HOTTINGER DG, et al. Anti- nervegrowth factor in pain management ;currentevidence. Pain Res,2016,9:373-383[2017-01-22]. https;//www. ncbi. nlm. nib. gov/pmc/articles/PMC4908933/, doi: 10. 2147/JPR. S89061.

DONE JD, RUDICK CN, QUICK ML, et al. Role of mast cells inmale chronic pelvic pain. J Urol, 2012, 187 ( 4 ): 1473- 1482.

MARTIN EZ- ROJ AS VA. BARRAGAN-IGLESIAS P. ROCHA-GONZALEZ HI,et al. Role of TRPV1 and ASIC3 informalin-induced secondary allodynia and hyperalgesia. Pharmacol Rep.2014.66(6) :964-971.

SLUKA KA, RASMUSSEN LA,EDGAR MM. Acid-sensing ion channel3 deficiency increases inflammation but decreases pain behavior in murine arthritis. Arthritis Rheum. 2013, 65 (5):1194-1202.


Refbacks

  • There are currently no refbacks.