Inflammatory Factors Promote the Expression of PD-L1 in Tca8113

LU Li-bing, CHEN Jiao, FENG Yun, ZHOU Chen-chen, ZHANG Ping

Abstract

To investigate the mechanism of immunologic escape in the tumor microenvironment, study the expression of programmed death 1 ligand-1 (PD-L1) in Tca8113 with treatment of inflammatory factors. Methods The expression of PD-L1 treated with inflammatory factors (IL-1β, IL-2, IL-6, TNF-α, IFN-γ) was detected by flow cytometry (FCM). Results The expression of PD-L1 in Tca8113 was up-regulated conspicuously with treatment of inflammatory factors (P<0.05), including:IL-1β, IL-6, TNF-α, IFN-γ. And the factors played the role in synergistic effects, most significantly in the groups of IL-1β+IFN-γ, TNF-α+IFN-γ and IL-1β+IL-6+IFN-γ+TNF-α. But the influence of IL-2 on the expression of PD-L1 was not significant (P>0.05). Conclusion With the up-regulated expression of PD-L1, the tumor would be escaped more easily from the immunoreactions, while there were an abundance of inflammatory factors in the tumor microenvironment.

 

Keywords: Inflammatory factors, PD-L1, Tca8113

 

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References


Hussain SP. Harris CC. Inflammation and cancer; an ancient link with novel potentials. Int J Cancer, 2007 ; 121 ( 11); 2373- 2380.

Ravetch JV, Lanier LL. Immune inhibitory receptors. Science, 2000; 290(5489): 84-89.

Dong H. Strome SE, Salomao DR, et al. Tumor associated B7-H1 promotes T cell apoptos is; a potential mechanism of immune evasion. Nat Med,2002;8(8) ;793-800.

Geng L, Huang D. Liu J, et at. B7-H1 up-regulated expression in human pancreatic carcinoma tissue associates with tumor progression. J Cancer Res Clin Oncol, 2008; 134 ( 9): 1021-1027.

Krambeck AE, Dong H, Thompson RH, et al. Survivin and B7-H1 are collaborative predictors of survival and represent potential therapeutic targets for patients with renal cell carcinoma. Clin Cancer Res,2007; 13(6); 1749-1756.

Shacter E, Weitzman SA. Chronic inflammation and cancer. Oncology, 2002; 16(2): 217-226.

Chen JJ , Lin YC, Yao PL. Tumor associated macrophages; the double edged sword in cancer progression. J Clin Oncol. 2005; 23(5):953-964.

Leek RD. Harris AL. Tumor-associated macrophages in breast cancer. J Mammary Gland Biol Neoplasia,2002;7(2); 177-189.

Nakayama Y, Nagashima N, Minagawa N, el al. Relationships between tumor-associated macrophages and clinicopathological factors in patients with colorectal cancer. Anticancer Res,2002;229(6C) : 4291-4296.

Hagemann T, Wilson J, Kulbe H. et al. Macrophages induce invasiveness of epithelial cancer cells via NF-кВ and JNK. J Immunol, 2005; 175(2): 1197-1205.

Hagemann T, Robinson SC, Schulz M, et al. Enhanced invasiveness of breast cancer cell lines upon co-cultivation with macrophages is due to TNF-a dependent up-regulation of matrix metallo proteases. Carcinogenesis, 2004 ; 25 (8) ; 1543- 1549.

Pukrop T, Klemm F, Hagemann T, et al. Wnt 5a signaling is critical for macrophage induced invasion of breast cancer cell lines. Proc Natl Acad Sci U S A,2006; 103(14) :5454-5459.

Paul PT. Gary SF. NF-кВ: a key role in inflammatory diseases. J Clin Invest,2001; 107( 1) :7-l 1.

Greten FR, Eckmann L. Greten TF. et al. IKK|3 links inflammation and tumorigenesis in a mouse model of colitis- associated cancer. Cell, 2004 ; 118(3):285-296.

Coussens LM. Werb Z. Inflammation and cancer. Nature. 2002;420(6917):860-867.


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