Effects of Gelatin on Performance of α-tricalcium Phosphate Bone Cement

XIAO Li-juan, YANG Zheng, MAN Yi. et al

Abstract

To determine the effects of gelatin on the performance of calcium phosphate cement (CPC). Methods α-tricalcium phosphate (α-TCP) bone cement was mixed with different concentrations of gelatin solutions. The CPC samples were soaked into simulated body fluid for one day before their compressive and bending strengths were measured. We also compared their waterproof performance, solidification time and surface topography. Results gelatin solutions changed the performance of CPC. Optimal performance of CPC was achieved when the volume ratio of gelatin solution to CPC (Vgelatin solution∶VCPC liquid) was set at 25∶100,which increased compressive strength from (7.874 54±0.660 97) MPa to (9.936 52±0.433 17) MPa and bending strength from (5.157 06±0.298 30) MPa to (7.959 71±0.281 63) MPa. gelatin solution also prolonged setting time of CPC, improved its waterproof performance, and promoted formulation of more dense and uniform hydroxyapatite crystals. Conclusion Gelatin can improve the compressive and bending strengths of CPC, and make CPC more suitable for clinic use through improvements in setting time and waterproof performance.

 

Keywords: Calcium phosphate cement, Gelatin, Setting time, Waterproof performance, Strength 

 

Full Text:

PDF


References


VARALAKSHMI PR, KAVITHA M, GOVINDAN R, et al. Effect of statins with a-tricalcium phosphate on proliferation, differentiation, and mineralization of human dental pulp cells. J Endod.2013,39(6);806-812.

MARUKAWA K, UEKI K, OK ABE K, et al. Use of self¬setting a-tricalcium phosphate for maxillary sinus augmentation in rabbit. Clin Oral Implants Res,2011,22(6): 606-612.

ROJBANI H, NYAN M, OHYA K. et al. Evaluation of the osteoconductivity of a-tricalcium phosphate, p-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. J Biomed Mater Res A, 2011,98(4):488-498.

VARALAKSHMI PR, KAVITHA M, GOVINDAN R, et al. Effect of statins with a-tricalcium phosphate on proliferation, differentiation, and mineralization of human dental pulp cells. J Endod.2013,39(6);806-812.

BIGI A, PANZAVOLTA S, RUBINI K. Relationship between triple-helix content and mechanical properties of gelatin films. Biomaterials,2004 ,25(25) :5675-5680.

TOUNY AH, LAURENCIN C, NAIR L, et al. Formation of composites comprised of calcium deficient HAp and cross- linked gelatin. J Mater Sci: Mater Med. 2008, 19( 10) ; 3193- 3201.

KUIJPERS AJ, ENGBERS GH, KRIJGSVELD J. et al. Cross-linking and characteri sterisation of gelatin matrices for biomedical applications . Biomater Sci Polym Ed,2000,11(3) : 225-243.

MAO JS,CUI YL,WANG XH, et al. A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis. Biomaterials,2004,25(18):3971-3981.

BIGI A, BRACCI B, PANZAVOLTA S. Effect of added gelatin on the properties of calcium phosphate cement. Biomaterials,2004,25(14):2893-2899.

DOI Y, TAKEZAWA Y, SHIBATA S, et al. Self-setting apatite cement, in physicochemical properties. J Japan Soc Dent Matals Devices, 1987(6);53-58.

QU Y, YANG Y, LI J, et al. Preliminary evaluation of a novel strong/osteoinductive calcium phosphate cement. J Biomater Appl.2011.26(3) s311.

YUAN H, LI Y, DE BRUIJN JD, et al. Tissue responses of calcium phosphate cement; a study in dogs. Biomaterials, 2000,21(12):1283-1290.

PANZAVOLTA S, TORRICELLI P. BRACCI B. et al. Functionalization of biomimetic calcium phosphate bone cements with alendronate. Inorg Biochem, 2010, 104 ( 10); 1099-1106.

LEROUX L, HATIM Z,FRECHE М .et al. Effect of various adjuvants ( lactic acid, glycerol and chitosan ) on the injectability of a calcium phosphate cement. Bone, 1999,25( 2 Suppl) :31S-34S.

MISTRY AS, MIKOS AG. Tissue engineering strategies for bone regeneration. Adv Biochem Eng Biotechnol, 2005 (94) ; 1-22.

HUTMACHER DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials,2000,21 (24);2529-2543.

DURUCAN C, BROWN PW. alpha-tricalcium phosphate hydrolysis to hydroxyapatite at and near physiological temperature. J Mater Sci Mater Med,2000,11 (6):365-371. MONMA H, KANAZAWA T. The hydration of crtricalcium phosphate. J Cera Soc Jap, 1976 ,84(4) ;209-213.

JANSEN J,OOMS E, VERDONSCHOT N,et al. Injectable calcium phosphate cement for bone repair and implant fixation. Ortho Clin N Am,200o ,3b( 1) ;89-9o.

PANZAVOLTA S, TORRICELLI P, STURBA L, et al. Setting properties and in -vitro bioactivity of strontium- enriched gelatin-calcium phosphate bone cements. J Biomed Mater Res A,2008,84(4):965-972.

CARRODEGUAS RG, DE AZA S. a-tricalcium phosphate; synthesis, properties and biomedical applications. Acta Biomater.2011.7(10) :3536-3546.

MIYAMOTO Y.ISHIKAWA К. ТА К ЕС HI М. et al. Non¬decay type fast-setting calcium phosphate cement; setting behaviour in calf serum and its tissue response. Biomaterials, 1996»17(14);1429-1435.

ISHIKAWA К. MIYAMOTO Y. KON M. et al. Non-decay type fast-setting calcium phosphate cement: composite with sodium alginate. Biomaterials, 1995,16(7):527-532.


Refbacks

  • There are currently no refbacks.