Effect of Electroacupunctrue on ERp57 in NAFLD Rats

ZHANG Yi, TANG Cheng-lin, TIAN Yuan. et al

Abstract

To investigate the effect of electroacupunctrue on ERp57 in non-alcoholic fatty liver disease (NAFLD) rats, and study the therapeutic mechanism of electroacupunctrue in patients with NAFLD. Methods Sixty male SD rats were randomly divided into common diet group (n=15) and high-fat diet group (n=45). 5 weeks later, two rats from the two groups were executed and confirmed that the model was successful. Then 10 rats in common diet group were chosen as control group (Control), and 40 rats in high-fat diet group were randomly chosen and divided into diet group 1 (D1), diet group 2 (D2), electroacupuncture group 1 (EA1) and electroacupuncture group 2 (EA2) (n =10 each). D1 and EA1 were fed by high fat diet; D2 and EA2 were fed with common diet. In EA1 and EA2, filiform needle acupuncture was applied to ST36, SP6 and Liv3 and electroacupunctrue was applied to one-side of ST36, SP6 for 20 min once daily for 4 weeks. The rats in each group were weighed per-week. After the treatment the changes of blood lipid and liver functions of these rats were observed. ERp57 gene expression and protein expression were detected by RT-PCR and Western blot, and expression of ERp57 downstream SREBP-1c was detected. Results The body mass of D1 increased slowly and were lower than D2 and EA1 ( P<0.05); the body weights of EA2 increased rapidly and were higher than EA1 ( P<0.05), but without significant difference with D2 ( P>0.05). The contents of blood lipid, liver functions and the expression of ERp57 and SREBP-1c were significantly higher than those in Control, D2 and EA1 ( P<0.05). While compared to D2 and EA1 respectively, the index mentioned above in EA2 decreased more significantly ( P<0.05). Conclusion Electroacupunctrue can decrease expression of ERp57 to improve endoplasmic reticulum stress (ERS) of rats with NAFLD and then decrease expression of SREBP-1c to regulate rat lipid, which could be one of mechanism to cure NAFLD.

 

Keywords: NAFLD, Electroacupunctrue, ERS ERp57 

 

 

Full Text:

PDF


References


Loomba R.Sanyal AJ. The global NAFLD epidemic. Nat Rev Gastroenterol Hepatol,2013; 10( 11) :686-690.

Ron D, Walter P. Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol, 2007;8(7);519-529.

Zhang Y.Venugopal SK.He S,et al. Ethanol induces apoptosis in hepatocytes by a pathway involving novel protein kinase С isoforms. Cell Signal,2007; 19( 11):2339-2350.

Liu J, Jin X, Yu CH, et al. Endoplasmic reticulum stress involved in the course of lipogenesis in fatty acids-induced hepatic steatosis. J Gastroenterol Hepatol, 2010; 25 ( 3); 613- 618.

Leamy AK,Egnatchik RA. Young JD. Molecular mechanisms and the role of saturated fatty acids in the progression of non¬alcoholic fatty liver disease. Prog Lipid Res, 2013 ; 52 ( 1); 165-

Wang H.Chan PK, Pan SY, et al. ERp57 is up-regulated in freefatty acids-induced steatotic L-02 cells and human nonalcoholic fatty livers. J Cell Biochem, 2010; 110 ( 6 ); 1447- 1456.

McPherson R, Gauthier A. Molecular regulation of SREBP function; the Insig-SCAP connection and isoform-specific modulation of lipid synthesis. Biochem Cell Biol. 2004 ; 82(1); 201-211.

Higuchi N, Kato M. Shundo Y, et al. Liver X receptor in cooperation with SREBP-lc is a major lipid synthesis regulator in nonalcoholic fatty liver disease. Hepatol Res, 2008; 38 ( 11) : 1122-1129.

Schwab ME. Repairing the injured spinal cord. Science.2002; 295(5557):1029-1031.

Marillat V, Cases O, Nguyen-Ba-Charvet KT, et al. Spatiotemporal expression patterns of slit and robo genes in the rat brain. J Comp Neurol,2002;442(2): 110-155.

Seeger M. Tear G, Ferres-Marco D, et al. Mutations affecting growth cone guidance in Drosophila; genes neces-sary for guidance toward or away from the midline. Neuron. 1993; 10 (3);409-426.

Kaneko S, Iwanami A, Nakamura M, et al .A selective SemalA inhibitor enhances regenerative responses and functional recovery of the injured spinal cord. Nat Med,2006; 12(12):1380-1389.

Zheng B, Lee JK, Xie F. Genetic mouse models for studying inhibitors of spinal axon regeneration. Trends Neurosci, 2006; 29(11):640-646.

Whitford KL. Marillai V. Stein E. eial. Regulation of cortical dendrite development by Slit-Robo interactions. Neuron. 2002; 33(1) : 4 7-61.

Andrews W. Liapi A, Plachez C, et al. Robol regulates the development of major axon tracts and interneuron migration in the forebrain. Development ,2006; 133(11);2243-2252.

Johnson KG, Tenney AP, Ghose A, et al. The HSPGs Syndecan and Dallylike bind the receptor phosphatase LAR and exert distinct effects on synaptic development. Neuron,2006;49(4)j517-531.

Walz A, McFarlane S, Brickman YG, et al. Essential role of heparan sulfates in axon navigation andtargeting in the developing visual system. Development, 1997; 124 ( 12): 2421- 2430.

Paveliev M,Hienola A.Jokitalo Y.et al. Sensory neurons from N-syndecan-deficient mice are defective in survival. Neuroreport.2008; 19( 14 ): 1397-1400.


Refbacks

  • There are currently no refbacks.