Expression Changes of IL-1β in Lung of Rats Subjected to Brain Ischemia

YUE Qian-yu, DAN Qi-qin, RONG Rong.

Abstract

To study the expression changes of interleukin-1β (IL-1β) in the lung of rats with brain ischemia. Methods Adult SD rats were divided into sham operation group and brain ischemia lung injury (BILI) group randomly. Focal cerebral ischemia inflammatory lung injury model was developed with intraluminal thread technique. Lungs were harvested from rats at different time point respectively. RT-PCR (24 h), Western blot (48 h) and immunohistochemistry (72 h) were employed to detect the expressional changes and the distributions of IL-1β in the lung tissues. Results IL-1β immunohistochemical positive reaction was observed in epithelia cell and neutrophil as well as macrophage. Increased protein level and mRNA expression for IL-1β were found in lung after brain ischemia compared with those of sham group. Conclusion IL-1β, as a crucial inflammatory factor, could be associated with airway inflammation in lung following brain ischemia in rats.

Keywords: Brian ischemia lung injury IL-1β Rat 

 

Full Text:

PDF


References


Cohan VL. Scott AL, Dinarello С A, el al. Interleukin-1 is a mucus secretagogue. Cell Immunol, 1991; 136(2) :425-434.

Sapey E, Ahmad A, Bayley D. el al. Imbalances between interleukin-1 and tumor necrosis factor agonists and antagonists in stable COPD. J Clin Immunol,2009;29(4);508-516.

Chaouat A, Savale L, Chouaid С, el al. Role for interleukin-6 in COPD-related pulmonary hypertension. Chest, 2009; 136 (3) ;678-687.

Yang HJ, Yang XY, Ba YC, el al. Role of Neurotrophin 3 in spinal neuroplasticity in rats subjected to cord transection. Growth Factors,2009;27(4) : 237-246.

Xiyang YB. Liu S, Liu J, et al. Roles of platelet-derived growth factor-B expression in the ventral horn and motor cortex in the spinal cord-hemisected rhesus monkey. J Neurotrauma,2009; 26(2): 275-287.

Li XL, Zhang W, Zhou X, et al. Temporal changes in the expression of some neurotrophins in spinal cord transected adult rats. Neuropeptides,2007;41 (3); 135-143.

Qin DX. Zou XL, Luo W, et al. Expression of some neurotrophins in the spinal motoneurons after cord hemisection in adult rats. Neurosci Lett»2006;410(3) :222-227.

DenesA, Pinteaux E, Rothwell NJ, et al. Interleukin-1 and stroke; biomarker, harbinger of damage, and therapeutic target. Cerebrovascular Diseases,2011;32(6); 517-527.

Skifter DA, Allegrini PR, Wiessner C, et al. Similar time- course of interleukin-1 beta production and extracellular-signal- regulated kinase ( ERK ) activation in permanent focal brain ischemic injury. Metab Brain Dis,2002; 17(3); 131-138.

Couillin I, Vasseur V, Charron S, et al. IL-lRl/MyD88 signaling is critical for elastase-induced lung inflammation and emphysema. J Immunol.2009; 183(12) ;8195-8202.

MatoN.Fujii M, Hakamata Y, etal. Interleukin-1 receptor- related protein ST2 suppresses the initial stage of bleomycin- induced lung injury. Eur Respir J ,2009;33(6); 1415-1428.

Herold S.Tabar TS. Janssen H. et al. Exudate macrophages attenuate lung injury by the release of IL-1 receptor antagonist in gram-negative pneumonia. Am J Respir Crit Care Med. 2011;183(10):1380-1390.


Refbacks

  • There are currently no refbacks.