Changes of the Expression of Brain Derived Neurotrophic Factors in Rats Trachea Induced by Acrolein Exposure

YUAN Bing, YANG Rui-an, ZHAO Wei. et al

Abstract

To investigate expressional changes of brain derived neurotrophic factor (BDNF) in the trachea of rats with acrolein inhalation. Methods Twenty two SI) rats were divided into 2 groups; the rats in experimental group were subjected to acrolein inhalation for the induce of trachea inflammatory injury, while the rats with saline (NS) inhalation were as control. All the rats were sacrificed in 1,3,6 weeks after acrolein (rt= 11 at each time point) or saline inhalation (?/=ll at each time point), the samples of trachea epithelium were harvested. The immunohistochemistry and in situ hybridization was performed to detect the location of BDNF protein and mRNA in trachea. The expression of BDNF mRNA in the trachea tissues were determined by RT-PCR. Results There are positive cells in epithelium of trachea for BDNF protein and mRNA, with cytoplasm staining. The expression of BDNF mRNA in the trachea was increased at 1 week after acrolein inhalation (P<0. 05, vs. control group), then decreased along with the time and reached to the same level as control group at 3 weeks, then last to 6 weeks (P> 0.05). Conclusion The inflammatory injury in trachea induced by acrolein exposure could be associated with the increased expression of BDNF. BDNF may be one of the crucial inflammatory factors in the process of inflammatory reaction in trachea with acrolein stimulation.


Keywords: Acrolein inhalation, Trachea injury, BDNF, Rats

 

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References


Kehrer JH, Biswal SS. The molecular effects of aerolein. Toxicol Sci, 2000; 57 ( 1): 6-15.

Leikauf GD. Hazardous air pollutants and asthma. Environ Health Perspect*2002; 110(Suppl 4):505-526.

Borchers MT, Wesselkamper S, Wert SE, et al. Monocyte inflammation augments acrolein-induced Muc5ac expression in mouse lung. Am J Physiol, 1999 ;277(3 Pt 1): L489-L497.

Borchers MT. Wesselkamper SC. Harris NL. et al. CD8(t) T cells contribute to macrophage accumulation and airspace enlargement following repeated irritant exposure. Exp Mol Pathol, 2007;83(3): 301-310.

Yan Q. Rosenfeld RD, Matheson CR, et al. Expression of brain derived neurotrophic factor protein in the adult rat central nervous system. Neuroscience, 1997;78(2);431-448.

Conner JM. Lauterborn JC, Yan Q, et al. Distribution of BDNF protein and mRNA in the normal adult rat CNS. J Neurosci, 1997; 17(7);2295-2313.

Griesbach GS, Hovda DA, Molteni R. et al. Voluntary exercise following traumatic brain injury: brain-derived neurotrophic factor upregulation and recovery of function. Neuroscience, 2004; 125( 1): 129-139.

Rostami E, Krueger F, Plantman S, et al. Alteration in BDNF and its receptors, full-length and truncated TrkB and p75(NTR) following penetrating traumatic brain injury. Brain Res,2014; 1542: 195-205. doi: 10. 1016/j. brainres. 2013. 10. 047.

Lommatzsch МЛ, Braun A, Mannsfeldt VA, et al. Abundant production of brain-derived neurotrophic factor by adult visceral epithelia. Implications for paracrine and target-derived Neurotrophic functions. Am J Pathol, 1999; 155 ( 4 ): 1183- 1193.

Tessarollo L. Pleiotropic functions of neurotrophins in development. Cytokine Growth Factor Rev, 1998; 9 ( 2 ) : 125- 137.

Sariola H. The neurotrophic factors in non-neuronal tissues. Cell Mol Life Sci,2001 ;58(8): 1061-1066.

Vega J A, Garcia-Sudrez O, Hannestad J , et al. Neurotrophins and the immune system. J Anat,2003;203( 1): 1-19.

Leikauf GD, McDowell SA, Wesselkamper SC, et al. Pathogenomic mechanisms for particulate matter induction of acute lung injury and inflammation in mice. Res Rep Health Eff Inst,2001;105(12):5-58.

Yang HJ, Yang XY, Ba YC, et al. Role of Neurotrophin 3 in spinal neuroplasticity in rats subjected to cord transection. Growth Factors,2009;27(4) ;237-246.

Wang XY. Li XL, Hong SQ. et al. Electroacupuncture induced spinal plasticity is linked to multiple gene expressions in dorsal root deafferented rats. J Mol Neurosci, 2009; 37( 2): 97-110.

Chen J. Qi JQ, Zhang W, et al. Electro-acupuncture induced NGF, BDNF and NT-3 expression in spared L6 dorsal root ganglion in cats subjected to removal of adjacent ganglia. Neurosci Res, 2007 ; 59(4): 399-405.

Kemi С, Grunewald J, Eklund A, et al. Differential regulation of neurotrophin expression in human bronchial smooth muscle cells. Respir Res,2006;7:18.

Braun A, Lommatzsch M, Mannsfeldt A, et al. Cellular sources of enhanced brain-derived neurotrophic factor production in a mouse model of allergic inflammation. Am J Respir Cell Mol Biol,1999;21 (4) ;537-546.

Garcia-Sudrez O, Perez-Pinera P, Laura R, et al. TrkB is necessary for the normal development of the lung. Respir Physiol Neurobiol, 2009; 167( 3); 281-291.

Sopi RB, Martin RJ, Haxhiu MA, et al. Role of brain-derived neurotrophic factor in hyperoxia-induced enhancement of contractility and impairment of relaxation in lung parenchyma. Am J Physiol Lung Cell Mol Physiol, 2008; 295 ( 2); L348- L355.

Noga O, Hanf G, Schaper C, et al. The influence of inhalative corticosteroids on circulating Nerve Growth Factor, Brain- Derived Neurotrophic Factor and Neurotrophin-3 in allergic asthmatics. Clin Exp Allergy,2001;31 (12): 1906-1912.


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