Effect of HIF-1α Gene Silence on Biological Characteristics of Human Colon Cancer Cells SW480

WANG Qiang, HAO Lang-song, SHI Jia. et al

Abstract

To investigate changes in proliferation and apoptosis of human colon cancer SW480 cells after silencing hypoxia inducible factor-la (HIF-la) expression. Methods siRNA interference technology was performed to silence the expression of HIF-\a using lipofectamine mediation to transfect siRNA into human colon cancer SW480 cells. The siRNA interfered SW480 cells were compared with a negative control group, an empty vector group, and a blank control group. Real-time PCR and Western blot were used to measure the expressions of HIF-la protein and mRNA. MTT and flow cytometry (FCM) were used to evaluate the apoptosis and proliferation of SW480 cells. Results The interfered SW480 cells had a higher level of silence of HIF-\a mRNA 0 80%) compared with those of in the three control groups (P<0. 05). Higher levels of apoptosis and lower levels of proliferation were detected in the interfered cells compared with those in the control groups (P<0.01). Conclusion HIF-la silencing promotes apoptosis and inhibits the proliferation of SW480 cells in vitro.

 

Keywords: Hypoxia inducible factor-la, RN A interference, Colon cancer cells, Proliferation, Apoptosis

 

Full Text:

PDF


References


Semenza GL. Defining the role of hypoxia-inducible factor 1 cancer biology and therapeutics. Oncogene, 2010; 29 ( 5 ); 625- 634.

Rajaganeshan R. Prasad R, Guillou PJ, et al. The role of hypoxia in recurrence following resection of Dukes' В colorectal cancer. Int J Colorectal Dis,2008;23( 11): 1049-1055.

Schmitz KJ, Muller Cl, Reis H, et al. Combined analysis of hypoxia inducible factor 1q and metallothionein indicates an aggressive subtype of colorectal carcinoma. Int J Colorectal Dis, 2009;24(11):1287-1296.

Cleven AH. van Engeland M, Wouters BG, et al. Stromal expression of hypoxia regulated proteins is an adverse prognostic factor in colorectal carcinomas. Cell Oncol. 2007; 29 ( 14 ): 229- 240.

Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell,2011; 144(5):646-674.

Kim JW. Tchernyshyov I, Semenza GL, et al. HIF-1-mediated expression of pyruvate dehydrogenase kinase;a metabolic switch required for cellular adaptation to hypoxia. Cell Metab.2006;3 (3):177-185.

Ward PS, Thompson CB. Metabolic reprogramming: a cancer hallmark even Warburg did not anticipate. Cancer Cell,2012;21 (3):297-308.

Lum JJ, Bui T, Gruber M, etal. The transcription factor HIF- 1 alpha plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev, 2007;21(9):1037-1049.

Kola L, Landis J. Can the pharmaceutical industry reduce attrition rates? Nat Rev Drug Discov.2004 ;3С8): 1774-1776.


Refbacks

  • There are currently no refbacks.