Effect of Recombinant Super-compound Interferon (rSIFN-co) on Proliferation and Apoptosis of Human Pulmonary Adenocarcinoma Cell A549

KAN Qi-wei, LIU Lun-xu, WEI Guang-wen. et al

Abstract

To investigate the effect of recombinant super-compound interferon (rSIFN-co) on the proliferation and apoptosis of pulmonary adenocarcinoma cell line A549. Methods Screening tests were conducted to determine the concentrations of rSIFN-co that have a significant impact on A549 and the optimal concentration and duration for the test of rSIFN-co combined with Cisplatin. A549 cells were treated with rSIFN-co. Infergen. rSIFN- co4- Cisplatin. Infergen + Cisplatin. and Cisplatin. respectively, and compared with those cultured in normal medium. The viable A549 cells from Day 1 to Day 7 were detected by MTT assay. Cell apoptosis was detected by- flow cytometry (FCM). Apoptosis-associated proteins* Fas and Iicl-2 were detected by immunofluoroscence at 48 h. Results Effective concentrations of rSIF.\-co ranged from 1 to 64 ^tg/mL, and a minimal of 2 ptg/mL Cisplatin was needed. The optimal test condition was set at 5 jig/mL rSIFNI-co combined with 2 ^g/mL Cisplatin for a duration of 48 h. rSIFN-co demonstrated a stronger inhibiting effect on cell proliferation than Infergen. The inhibiting efficiency of rSIFN-co+Cisplatin was also stronger than that of Infergen + Cisplatin. Apoptosis of A549 cells induced by rSIFN-co was also more significant than that of Infergen (P = 0. 000). Cells treated with rSIFN- co+ Cisplatin has a higher apoptosis rate than those treated with rSIFN-co (P = 0. 004) or Cisplatin (P = 0. 023). rSIFN-co increased the expression of Fas and decreased the expression of Bel 2. Cells treated with rSIFN-co showed lower fluoroscence intensity of Iicl-2 than those treated with Infergen (P< 0. 05). Conclusion rSIFN-co inhibits the proliferation of A549 and its effect is stronger than that of Infergen. Cisplatin can further enhance the inhibiting effect of rSIFN со. The inhibiting efficiency may be associated with the expression of apoptosis-related genes.

 

Keywords: Recombinant super-compound interferon ( rSIFNI-co) Pulmonary adenocarcinoma Proliferation Apoptosis


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References


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