Sodium Ferulate Attenuates Oxidative Stress Induced Inflammation via Suppressing NALP3 and NF-κB Signal Pathway
Abstract
To study the effects of sodium ferulate on inflammation in human lung epithelial cells (A549) under oxidative stress and itsinfluence onthe expression of inflammasome NACHT-PYD-containing protein 3 (NALP3) and nuclear factor kappa B (NF-κB). Methods Human lung epithelial cells A549 cultured In vitro were divided into 4 groups, including control group, H 2 O 2 (100 μmol/L) stress group, NF-κB blockers group (PDTC 100 μmol/L+ H 2 O 2 100 μmol/L), sodium ferulate （SF） intervention group (SF 400 μg/mL+H 2 O 2 100 μmol/L). The expression of NALP3, IκBα protein were evaluated by Western blot, while mRNA levels of NALP3,NF-κB（P65） were measured by qRT-PCR.The level of interleukin-1beta (IL-1β) were detected by ELISA. Results H 2 O 2 not only increased the mRNA and protein expression levels of NALP3, but also enhanced the secretion of IL-1β in human lung epithelial cells A549 (P<0.05) when compared with control group.NF-κB blockers PDTC and sodium ferulateresisted the effects of H 2 O 2 on A549 cells, that decreased the mRNA and protein expression of NALP3 and the mRNA expression of NF-κB (P65), reduced the degeneration of IκBα and the secretion of IL-1β (P<0.05) when compared to H 2 2O 2 stress group. Conclusion SF may reduce the expression of NALP3 and IL-1β by inhibiting NF-κB, so as to reduce the inflammation caused by oxidative stress.
Keywords: H2O2, Human lung epithelial cells A549, NACHT-PYD-containing protein 3, Nuclear factor kappa B, Interleukin-1beta, Sodium ferulate
Full Text:
PDFReferences
Liu R. Ahmed KM. Nantajit D, et al. Therapeutic effects of alpha-lipoic acid on bleomycin-induced pulmonary fibrosis in rats. Int J Mol Med.2007; 19(6) ;865-873.
Wang DJ. Zhou WD, Dai XJ. et al. Study on effect and mechanism of sodium ferulate in preventing and treating ozone induced lung injury in mice. Chin J Integr Med, 2007; 13 ( 3 ): 211-214.
Wu ZJ. Yu J, Fang QJ, et al. Sodium ferulate protects against daunorubicin-induced cardiotoxicity by inhibition of mitochondrial apoptosis in juvenile rats. J Cardiovasc Pharmacol,2014;63(4):360-368.
Zhang J. Chen J, Yang J, et al. Sodium ferulate inhibits neointimal hyperplasia in rat balloon injury model. PLoS One. 2014; 9 ( 1 ): e87561. doi: 10. 1371/journal, pone. 0087561. eCollection 2014.
Yuan X. Sun Y. Miao N. et al. The synergistic anti-inflammatory effect of the combination of sodium ferulate and oxymatrine and its modulation on inflammation-associated mediators in RAW 264.7 cells. J Ethnopharmacol, 2011; 137 (3);1477-1485.
Kummer J A, Broekhuizen R. Everett H , et al. Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response. J Histochem Cytochem, 2007;55(5) : 443-452.
Bauernfeind FG, Horvath G, Stutz A. et al. Cutting edge; NF- kB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression. J Immunol.2009; 183(2) : 787-791.
Segovia J . SabbahA, Mgbemena V, el at, TLR2/MyD88/NF- kB pathway, reactive oxygen species, potassium efflux activates NLRP3/ASC inflammasome during respiratory syncytial virus infection. PLoS One. 2012; 7( 1): e29695. doi: 10. 1371/journal, pone. 0029695. Epub 2012 Jan 25.
Chen L, Li X. Huang L. et al. Chemical stimulation of the intracranial dura activates NALP3 inflammasome in trigeminal ganglia neurons. Brain Res,2014; 1566 ; 1-11. doi; 10. 1016/j. hrainres. 2014. 04. 019. Epub 2014 Apr 23.
Wang CP, Zhang LZ, Li GC, et al. Mulberroside a protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion. J Neurosci Res,2014;92(7) :944-954.
Schroder K. Tschopp J. The inflammasomes. Cell, 2010; 140 (6):821-832.
Dostert C, Petrilli V, Van Bruggen R, et al. Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science,2008;320(5876) ;674-677.
Varfolomeev E, Goncharov T, Maecker H, et al. Cellular inhibitors of apoptosis by members of NF-kappaB and МАРК activation by member of the TNF family of receptors. Sci Signal,2012;5(216):ra22. doi; 10. 1126/scisignal. 2001878.
West AP, Brodsky IE, Rahner C, et al. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature,2011;472(7344):476-180.
Li FQ.Su H.Chen X, et al. Mannose 6-phosphate-modified bovine serum albumin nanoparticles for controlled and targeted delivery of sodium ferulate for treatment of hepatic fibrosis. J Pharm Pharm, 2009; 61 (9); 1155-1161.
Pei X . Wang W, Miao N. et al. The protective effects of the combination of sodium ferulate and oxymatrine on ethanol- induced liver damage in mice. Environ Toxicol Pharmacol, 2014;37(1);423-430.
Yang J. Xu SN, Wang al. Effects of sodium ferulate on liver fibrosis in experimental cirrhotic rats. World Chinese Journal of Digestology,2014 ;22( 12); 1676-1681.
Wang W. Pei X, Sun S. et al. Protective effects of the combination of sodium ferulate and oxymatrine on cecal ligation and puncture-induced sepsis in mice. Exp Ther Med, 2014; 7 (5):1297-1304.
Refbacks
- There are currently no refbacks.
