Investigate the Clonal Origin of Different Phenotypic Tumor-like Cells in the Epidermis and Dermis of Skin Lesions in Lymphomatoid Papulosis

WEN Peng-fei, WANG Ting-ting, WANG Lin. et al

Abstract

To explore the correlation of the clone sources of MF-like cells in the epidermis and RS-like cells in the dermis of lymphomatoid papulosis (LyP), a compartive study of this two type cells in 8 LyP cases was carried out. Methods 8 cases of LyP (4 type A cases and 4 type C cases) were detected the immunophenotype first, and then comparative study between the MF-like cells and RS-like cells was conducted on T-cell receptor (TCR) γ-chain gene rearrangement by combinating laser capture microdissectinon (LCM) and PCR. PCR products of one patient which had positive bands were selected randomly for sequencing and sequence alignment analysis. Results Monoclonal \TCRγ gene rearrangement was detected in 6 cases (75%) of the 8 cases. Detection rates of LyP type A and type C were 50% (2/4) and 100% (4/4), respectively. The rates of MF-like cells and RS-like cells were 75% (6/8) and 75% (6/8), respectively. Homology of the products with positive stripes in both MF-like cells and RS-like cells in one randomly selected case was compared and analyzed, and the results showed the homology of them was 106/108 (98%). Conclusion The MF-like cells in the epidermis and the RS-like cells in the dermis of LyP may be from the same clone.

 

Keywords:  Lymphomatoid papulosis, MF-like cells, RS-like cells, Clonal origin, Contrast study 


Full Text:

PDF


References


Willemze R. Jaffe ES, Burg G, et al. WHO-EORTC classification for cutaneous lymphomas. Blood.2005; 105(10): 3768-3785.

Swerdlow SH, Campo E, Harris NL. et al. WHO classification of tumours of haematopoietic and lymphoid tissues. Lyon: I ARC Press, 2008; 233-268.

El Shabrawi-Caelen L. Kerl H, Cerroni L. Lymphomatoid papulosis: reappraisal of clinicopathologic presentation and classification into subtypes A, B. and C. Arch Dermatol, 2004; 140(4):441-447.

Yang Н. Xu С, Tang Y. et al. The significance of multiplex PCR/heteroduplex analysis-based TCR-y gene rearrangement combined with laser-capture microdissection in the diagnosis of early mycosis fungoides. J Cutan Pathol.2012;39(3):337-346.

McCarthy KP, Sloane JP. kabarowski JH, et al. The rapid detection of clonal T-cell proliferations in patients with lymphoid disorders. Am J Pathol, 1991; 138(4) :821-828.

Weiss LM, Wood GS, Trela M, et al. Clonal T-cell populations in lymphomatoid papulosis. Evidence of a lymphoproliferative origin for a clinically benign disease. N Engl J Med. 1986;315(8):475-479.

Goeldet al, Cornillet-Lefebvre P, Durlach A, et al. T-cell receptor gamma gene rearrangement in cutaneous T-cell lymphoma; comparative study of polymerase chain reaction with denaturing gradient gel electrophoresis and GeneScan analysis. Br J Dermatol.2010; 162(4) ;822-829.

Alessi E, Coggi A, Venegoni L, et al. The usefulness of clonality for the detection of cases clinically and/or histopathologically not recognized as cutaneous T-cell lymphoma. Br J Dermatol.2005; 153(2);368-371.

Yazdi AS. Medeiros LJ, Puchta U, et al. Improved detection of clonality in cutaneous T-cell lymphomas using laser capture microdissection. J Cutan Pathol,2003;30(8) :486-490.

Gallardo F. Costa C, Bellosillo B. et al. Lymphomatoid papulosis associated with mycosis fungoides; clinicopathological and molecular studies of 12 cases. Acta Derm Venereol.2004; 84(6);463-468.

Greisser J, Palmedo G, Sander C, et al. Detection of clonal rearrangement of T-cell receptor genes in the diagnosis of primary cutaneous CD30 lymphoproliferative disorders. J Cutan Pathol, 2006;33(11):711-715.

Steinhoff M, Hummel M, Anagnostopoulos I, et al. Single¬cell analysis of CD30+ cells in lymphomatoid papulosis demonstrates a common clonal T-cell origin. Blood, 2002; 100 (2):578-584.

Gellrich S, Wernicke M. Wilks A, et al. The cell infiltrate in lymphomatoid papulosis comprises a mixture of polyclonal large atypical cells (CD30-positive) and smaller monoclonal T cells (CD30-negative). J Invest Dermatol,2004; 122(3);859-861.

Wang GB, Su JJ, Li LJ, et al. Edaravone alleviates hypoxia- acidosis/ reoxygenation- induced neuronal injury by activating ERK1/2. Neurosci Lett, 2013; 543; 72-77. doi; 10. 1016/j. neulet. 2013. 02. 067. Epub 2013 Apr.

Watanabe К, Ma M, Wen J. et al. Effects of edaravone in heart of aged rats after cerebral ischemia-reperfusion injury. Biol Pharm Bull,2007,30(3) :460-464.

Imai S. Inokuchi Y, Nakamura S. et al. Systemic administration of a free radical scavenger, edaravone, protects against light-induced photoreceptor degeneration in the mouse retina. Eur J Pharmacol,2010;642(1-3) ;77-85.

Inokuchi Y« Imai S, Nakajima Y, et al. Edaravone, a free radical scavenger, protects against retinal damage in -vitro and in vivo. J Pharmacol Exp Ther,2009;329(2) :687-698.

Kobayashi Y, Akamatsu Y, IwaneT, et al. Signaling pathway on the effect of oxygenated warm perfusion prior to cold preservation of the liver grafts from non-heart-beating donors, and the additive effect of edaravone. Transplant Proc,2009; 41 (1)-49-51.

Lee IT. Shih RH, Lin CC, et al. Role of TLR4/NADPH oxidase/ROS-activated p38 МАРК in VCAM-1 expression induced by lipopolysaccharide in human renal mesangial cells. Cell Commun Signal,2012; 10(1):33-48.


Refbacks

  • There are currently no refbacks.