Expression of OCT4 in Gastric Cancer Cell Lines and Its Significance

HUANG Zheng-jie, WANG Rui, LUO Wei-yuan

Abstract


Objective To detect the expression of octamer-binding protein-4（OCT4）in gastric cancer cell lines with different differentiation (MKN-28, SGC-7901, BGC-823) and normal gastric mucosal cells line GES-1, and further assess the relationship between OCT4 expression and the differentiation grade of gastric carcinoma cells. Methods Expression level of OCT4 in GES-1, MKN-28, SGC-7901 and BGC-823 was detected by reverse transcription-polymerase chain reaction (RT-PCR), and OCT4 siRNA was employed to interfere OCT4 expression in BGC-823 cell lines. Detect the quantity of OCT4 mRNA and OCT4 protein by fluorescent quantitative PCR and Western blot respectively. In addition, the invasion ability was analyzed via Transwell chamber. Results The normal gastric mucosal cells did not express OCT4 and there was higher expression of OCT4 in gastric cancer cell lines with poorly differentiation (P<0.05). The expression of OCT4 in BGC-823 cells was the highest. The expression of OCT4 mRNA and OCT4 protein were decreased distinctly in BGC-823 cells after being interfered by OCT4 siRNA (P<0.05). After being interfered by OCT4, BGC-823 cells were less aggressive, and the number of penetrating cells was decreased (P<0.05). Conclusion The OCT4 expression level is associated with gastric cancer differentiation. OCT4 may play an important role in the differentiation and invasion of gastric cancer cell and it may serve as a reference index in predicting the malignant grade of gastric cancer.

 

Keywords: Octamer-binding protein-4 gene, Gastric cancer cell lines RNAi, Malignant grade 

 

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References


Takahashi К. Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell,2006; 126(4);663-676.

Chambers I, Smith A. Self-renewal of teratocarcinoma and embryonic stem cells. Oncogene,2004 ;23(43); 7150-7160.

Park SW.Hu X,Gupta P, el al. SUMOylation of Tr2 orphan receptor involves Pml and fine-tunes Oct4 expression in stem cells. Nat Struct Mol Bio,2007 ; 1 (14); 68-75.

Atlasi Y, Mowla SJ, Ziaee SA, el al. OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer. Int J Cancer,2007;120(7):1598-1602.

Chen Z. Xu WR. Qian H« el al, Oct4, a novel marker for human gastric cancer. J Surg Oncol,2009;99(7) :414-419.

Miyoshi N, Ishii Н, Nagai К, el al. Defined factors induce reprogramming of gastrointestinal cancer cells. PNAS, 2010; 107(1): 40-45.

Lee J, Kim HK, Rho JY, el al. The human OCT-4 isoforms differ in their ability to confer self-renewal. J Biol Chem.2006; 281(44):33554-33565.

Wang X, Zhao Y. Xiao Z, el al. Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response. Stem Cells,2009;27(6); 1265-1275.


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