Feverfew Lactone Induces Autophagic Death of Hepatocellular Carcinoma SMMC 7721 Cells

LIU Zhan-pei, LI Yan-yan, GAO Bo. et al

Abstract

To explore the effect and mechanism of feverfew lactone on inducing autophagic death of hepatocellular carcinoma. Methods The proliferation of hepatocellular carcinoma SMMC 7721 cells treated with feverfew lactone was measured by MTT assay. The autophagy of SMMC 7721 induced with feverfew lactone was assessed by acridine orange staining, autophagic marker LC3 and p62 detecting and autophagic flows analyzing. In addition, a role of ROS in this process was stated by treatment with antioxidant agent N-acetyl-L-cysteine (NAC). Results The proliferation of SMMC 7721 cells were inhibited by feverfew lactone in a concentration dependence manner. The expression of LC3 and autophagic flows of SMMC 7721 cells were increased by feverfew lactone, while p62 was decreased, which implied that feverfew lactone could induce the autophagy of SMMC 7721 cells. Further more, the autophagy effect induced by feverfew lactone was declined obviously when traeted with NAC suggested that ROS played an important role in this effect. Conclusion Feverfew lactone induces autophagic death of SMMC 7721 cells by stimulating cells to produce ROS. The study will be helpful for the treatment of hepatocellular carcinoma and to provide theoretical basis for the clinical application of feverfew lactone.

 

Keywords: Feverfew lactone, Hepatocellular, Autophagy ROS 

 

Full Text:

PDF


References


Mizushima N. Methods for monitoring autophagy. Int J Biochem Cell Biol,2004;36( 12) ;2491-2502.

Mathema VB. Koh YS, Thakuri BC, et al. Parthenolide, a sesquiterpene lacton. expresses multiple anti-cancer and anti-inflammatory activities. Inflammation.2012;35(2):560-565. Knight DW. Feverfew; chemistry and biological activity. Nat Prod Rep»1995;12(3):271-276.

Garcia-Pineres AJ, Castro V, Mora G, et al. Cysteine 38 in p65/NF-kappaB plays a crucial role in DNA binding inhibition by sesquiterpene lactones. J Biol Chem, 2001; 276 ( 43 ); 39713- 39720.

Cheng G, Xie L. Parthenolide induces apoptosis and cell cycle arrest of human 5637 bladder cancer cells in vitro. Molecules, 2011;16(8):6758-6768.

Wang W, Adachi M, Kawamura R, et al. Parthenolide-induced apoptosis in multiple myeloma cells involves reactive oxygen species generation and cell sensitivity depends on catalase activity. Apoptosis,2006; 11 (12):2225-2235.

Xi G, Hu X, Wu B, et al. Autophagy inhibition promotes paclitaxel-induced apoptosis in cancer cells. Cancer Lett, 2011; 307(2):141-148.

Kongara S, Karantza V. The interplay between autophagy and ROS in tumorigenesis. Front Oncol,2012;2:171. doi: 10. 3389/ fonc. 2012. 00171. collection 2012.


Refbacks

  • There are currently no refbacks.