Effects of Thymosin α1 on Immune Effector Molecules of Mouse

Abstract

To analysis the effects of Tα1 on the immune effector molecules in mouse immune system. Methods Sixty five BABL/c mice were divided into four groups: CsA group (n=20), Tα1 group (n=20), CsA+Tα1 group (n=20) and control group (n=5). In the 3 experimental groups, 10 mg/kg CsA, 400 μg/kg Tα1, 10 mg/kg CsA+400 μg/kg Tα1 were respectively administrated by intraperitoneal injection daily. Luminex was performed for cytokine detection at 1 d, 7 d, 14 d, 21 d day after the above treatments. Lymphocyte culture was prepared with the mouse spleen suspension, and then treated with 0.25 mg/mL CsA, 10 μg/mL Tα1 or 0.25 mg/mL CsA+10 μg/mL Tα1 in vitro, respectively. Three days later, OD values of each treated lymphocyte culture and several cytokines in the culture were measured. Results Compared with other groups, CsA+Tα1 group had significant lower IL-1α, IL-2, IL-6, IL-17, and significant higher IL-10 at 1 d, 7 d, 14 d, 21 d after the treatments （P＜0.05）. Three days after the culture, OD value in the control group was significantly higher than that in Tα1 group, CsA group, and CsA+Tα1 group (P<0.05). IL-1α and IL-6 in the control group were significantly higher than those in the experiment groups (P<0.05), while IL-10 in the control group was significantly lower than that in the experiment groups (P<0.05). IL-2 and IL-17 were similar. Conclusion Tα1 show regulatory effect on the immune effector molecules which could promote Th1 cells transforming to Th2 cells.

Keywords: Thymosin α1, Cyclosporin, A Immune regulation