Gene Expression of The Efflux System AcrAB, OqxAB, and MarA Operons of Klebsiella Pneumoniae Isolated from Patients with Urinary Tract Infection
Abstract
Approximately 150 million individuals worldwide have urinary tract infections (UTIs), which are among the most prevalent bacterial illnesses. Klebsiella pneumoniae is a common opportunistic pathogen that causes nosocomial infections. The efflux pump genes (AcrAB, OqxAB, and MarA) were isolated from a variety of UTI patients under the supervision of a specialist physician at two healthcare facilities in the city of AL-Dewaniyah: AL-Dewaniyah General Teaching Hospital and AL-Dewaniyah Maternity and Pediatrics Teaching Hospital, which is the aim of the current study. This study examined the prevalence of Klebsiella pneumoniae bacteria that cause UTIs. A total of 36 isolates were identified as K. pneumoniae according to manual culture characteristics and confirmed using the Vitek-2 system. Using real-time PCR, efflux pump genes (AcrAB, OqxAB, and MarA) were expressed. We found a high rate of AcrAB gene expression of AcrAB (17.3%), which was observed in 36(100%) K. pneumoniae isolates.
Keywords: Klebsiella pneumoniae, urinary tract infections (UTIs), real-time polymerase chain reaction (PCR), AcrAB.
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DAGNE E, DOBO B, & BEDEWI Z. Antibacterial Activity of Papaya (Carica Papaya) Leaf and Seed Extracts against Some Selected Gram Positive and Gram-Negative Bacteria. Pharmacog. J, 2021, 13(6s): 1727-1733. doi: 10.5530/pj.2021.13.223
SERAH SO. Prevalence of Antibiotic Resistant Bacteria Isolated from Phones. Research project 19/6039. College of Pure and Applied Sciences, Caleb University Imota, Lagos, Nigeria, 2022.
ROE JM, SEELY K, BUSSARD CJ, et al. Hacking the immune response to solid tumors: harnessing the anti-cancer capacities of oncolytic bacteria. Pharmaceutics, 2023, 15(7): 2004. doi: 10.3390/pharmaceutics15072004
RAJESWARI S. A Study on phenotypic detection of carbapenemases in klebsiella pneumoniae and their molecular characterization from a tertiary care hospital. Doctoral dissertation, Madurai Medical College, Madurai, 2018.
MALIK TA. Genotypic Characterization of Antibiotic Resistant Klebsiella Species. Doctoral dissertation, University of Karachi, Karachi, 2018.
JOSEPH L, MERCIECCA T, FORESTIER C, BALESTRINO D, & MIQUEL S. From Klebsiella pneumoniae colonization to dissemination: an overview of studies implementing murine models. Microorganisms, 2021, 9(6): 1282. doi: 10.3390/microorganisms9061282
BENGOECHEA JA, & SA PESSOA J. Klebsiella pneumoniae infection biology: living to counteract host defences. FEMS Microbiol Rev, 2019, 43(2): 123-144, doi: 10.1093/femsre/fuy043
BHOWMIK A. Role of Diagnostic procedures in managing human Bacterial infections: A comprehensive overview. Arch Hematol Case Rep Rev, 2023, 8(1): 008-019, doi: 10.17352/ahcrr.000043
LYONS PG, & KOLLEF MH. Prevention of hospital-acquired pneumonia. Curr Opin Crit Care, 2018, 24(5): 370-378, doi: 10.1097/mcc.0000000000000523
JIAO J, YANG XY, LI Z, et al. Incidence and related factors for hospital-acquired pneumonia among older bedridden patients in China: a hospital-based multicenter registry data based study. Front Public Health, 2019, 7: 221, doi: 10.3389/fpubh.2019.00221
GUCLU E, HALIS F, KOSE E, et al. Risk factors of multidrug-resistant bacteria in community-acquired urinary tract infections. Afr Health Sci, 2021, 21(1): 214-219, doi: 10.4314/ahs.v21i1.28
ZHONG X, XU H, CHEN D, et al. First emergence of acrAB and oqxAB mediated tigecycline resistance in clinical isolates of Klebsiella pneumoniae pre-dating the use of tigecycline in a Chinese hospital. PloS One, 2014, 9(12): e115185m doi: 10.1371/journal.pone.0115185
LI J, ZHANG H, NING J, et al. The nature and epidemiology of OqxAB, a multidrug efflux pump. Antimicrob Resist Infect Control, 2019, 8: 1-13, doi: 10.1186/s13756-019-0489-3
MAURYA N, JANGRA M, TAMBAT R, & NANDANWAR H. Alliance of efflux pumps with β-lactamases in multidrug-resistant Klebsiella pneumoniae isolates. Microb Drug Resist, 2019, 25(8): 1155-1163, doi: 10.1089/mdr.2018.0414
ALBARRI O, ALMATAR M, ÖCAL MM, & KÖKSAL F. Overexpression of Efflux Pumps AcrAB and OqxAB Contributes to Ciprofloxacin Resistance in Clinical Isolates of K. pneumonia. Curr Protein Pept Sci, 2022, 23(5): 356-368, doi: 10.2174/1389203723666220630162920
BHARATHAM N, BHOWMIK P, AOKI, M, et al. Structure and function relationship of OqxB efflux pump from Klebsiella pneumoniae. Nat Commun, 2021, 12(1): 5400, doi: 10.1038/s41467-021-25679-0
LI Y, KUMAR S, ZHANG L, et al. Characteristics of antibiotic resistance mechanisms and genes of Klebsiella pneumoniae. Open Med, 2023, 18(1): 20230707, doi: 10.1515/med-2023-0707
HENNEQUIN C, & ROBIN F. Correlation between antimicrobial resistance and virulence in Klebsiella pneumoniae. Eur J Clin Microbiol Infect Dis, 2016, 35(3): 333-341, doi: 10.1007/s10096-015-2559-7
BLANCO, P, HERNANDO-AMADO, S, REALES-CALDERON, J. A, et al. Bacterial multidrug efflux pumps: much more than antibiotic resistance determinants. Microorganisms, 2016, 4(1): 14, doi: 10.3390/microorganisms4010014
AUDA IG, SALMAN IMA, & ODAH JG. Efflux pumps of Gram-negative bacteria in brief. Gene Rep, 2020, 20: 100666, doi: 10.1016/j.genrep.2020.100666
GOMES AÉI, STUCHI LP, SIQUEIRA NMG, et al. Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR. Sci Rep, 2018, 8(1): 9001, doi: 10.1038/s41598-018-27420-2
MIRZAIE, A, & RANJBAR, R. Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples. AMB Express, 2021, 11(1): 122, doi: 10.1186/s13568-021-01282-w
TANG M, WEI X, WAN X, et al. The role and relationship with efflux pump of biofilm formation in Klebsiella pneumoniae. Microb Pathog, 2020, 147, 104244, doi: 10.1016/j.micpath.2020.104244
KHALID TM, & GHAIMA KK. Molecular Detection of acrAB and oqxAB Genes in Klebsiella pneumoniae and Evaluation the Effect of Berberine on their Gene Expression. Iraqi J biotechnol, 2022, 21(2): 124-135.
XU Q, JIANG J, ZHU Z, et al. Efflux pumps AcrAB and OqxAB contribute to nitrofurantoin resistance in an uropathogenic Klebsiella pneumoniae isolate. Int J Antimicrob Agents, 2019, 54(2): 223-227, doi: 10.1016/j.ijantimicag.2019.06.004
VELEB, M, HIGGINS PG, GONZALEZ G, et al. Characterization of RarA, a novel AraC family multidrug resistance regulator in Klebsiella pneumoniae. Antimicrob Agents Chemother, 2012, 56(8): 4450-4458, doi: 10.1128/aac.00456-12
YOON EJ, OH Y, & JEONG SH. Development of tigecycline resistance in carbapenemase-producing Klebsiella pneumoniae sequence type 147 via AcrAB overproduction mediated by replacement of the ramA promoter. Ann Lab Med, 2020, 40(1): 15, doi: 10.3343/alm.2020.40.1.15
XU Q, SHENG Z, HAO M, et al. RamA upregulates multidrug resistance efflux pumps AcrAB and OqxAB in Klebsiella pneumoniae. Int J Antimicrob Agents, 2021, 57(2): 106251, doi: 10.1016/j.ijantimicag.2020.106251
BIALEK-DAVENET S, LAVIGNE JP, GUYOT K, et al. Differential contribution of AcrAB and OqxAB efflux pumps to multidrug resistance and virulence in Klebsiella pneumoniae. J Antimicrob Chemother, 2015, 70(1), 81-88, doi: 10.1093/jac/dku340
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